![]() Flexibility of eukaryotic Okazaki fragment maturation through regulated strand displacement synthesis. The Pol32 subunit of DNA polymerase δ contains separable domains for processive replication and proliferating cell nuclear antigen (PCNA) binding. Replication-dependent marking of DNA by PCNA facilitates CAF-1-coupled inheritance of chromatin. Multiple sequence-specific factors generate the nucleosome-depleted region on CLN2 promoter. Nascent transcript sequencing visualizes transcription at nucleotide resolution. Nucleosomes can form a polar barrier to transcript elongation by RNA polymerase II. High-resolution dynamic mapping of histone–DNA interactions in a nucleosome. An improved map of conserved regulatory sites for Saccharomyces cerevisiae. ![]() A library of yeast transcription factor motifs reveals a widespread function for Rsc3 in targeting nucleosome exclusion at promoters. Mechanisms that specify promoter nucleosome location and identity. Chromatin remodelling at promoters suppresses antisense transcription. A compiled and systematic reference map of nucleosome positions across the Saccharomyces cerevisiae genome. Conserved nucleosome positioning defines replication origins. OriDB: a DNA replication origin database. Gene identification signature (GIS) analysis for transcriptome characterization and genome annotation. Chromosomal ARS1 has a single leading strand start site. Heat-inducible degron and the making of conditional mutants. Discrete sizes of replication intermediates in Drosophila cells. Discrete start sites for DNA synthesis in the yeast ARS1 origin. Metabolism of Okazaki fragments during simian virus 40 DNA replication. Evidence that errors made by DNA polymerase α are corrected by DNA polymerase δ. ![]() Saccharomyces cerevisiae cell cycle mutant cdc9 is defective in DNA ligase. Idling by DNA polymerase δ maintains a ligatable nick during lagging-strand DNA replication. On the roles of Saccharomyces cerevisiae Dna2p and Flap endonuclease 1 in Okazaki fragment processing. I., Veeraraghavan, J., Polaczek, P., Campbell, J. RPA governs endonuclease switching during processing of Okazaki fragments in eukaryotes. Distribution of functions between FEN1 and DNA2. Anatomy of a DNA replication fork revealed by reconstitution of SV40 DNA replication in vitro. Two DNA polymerases may be required for synthesis of the lagging DNA strand of simian virus 40. ![]() An oligoribonucleotide polymerase from SV40-infected cells with properties of a primase. Polymerase dynamics at the eukaryotic DNA replication fork. The replication fork, core histone segregation and terminal structures. Structure of replicating simian virus 40 minichromosomes. Making copies of chromatin: the challenge of nucleosomal organization and epigenetic information. Our studies represent the first high-resolution analysis-to our knowledge-of eukaryotic Okazaki fragments in vivo, and reveal the interconnection between lagging-strand synthesis and chromatin assembly.Ĭorpet, A. ![]() Disrupting chromatin assembly or lagging-strand polymerase processivity affects both the size and the distribution of Okazaki fragments, suggesting a role for nascent chromatin, assembled immediately after the passage of the replication fork, in the termination of Okazaki fragment synthesis. Using deep sequencing, we demonstrate that ligation junctions preferentially occur near nucleosome midpoints rather than in internucleosomal linker regions. Here we show that ligation-competent Okazaki fragments in Saccharomyces cerevisiae are sized according to the nucleosome repeat. Eukaryotic Okazaki fragments remain poorly characterized and, because nucleosomes are rapidly deposited on nascent DNA, Okazaki fragment processing and nucleosome assembly potentially affect one another. Fifty per cent of the genome is discontinuously replicated on the lagging strand as Okazaki fragments. ![]()
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